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. 2022 Jun 11;27:45. doi: 10.1186/s11658-022-00351-7

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© The Author(s) 2022

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Fig. 2 — Characterization of CAFs isolated from patients with BC. A Representative pictures of immunofluorescence staining for α-SMA (red) in α-SMA^low and α-SMA^high CAFs; DAPI (blue) was used for nuclei counterstaining. B Representative pictures of MCF7 cells growing in 3D-Matrigel cultures, when treated with conditioned medium from α-SMA^low or α-SMA^high CAFs, and BJ or DMEM as a control. C Quantification of colony area fold change. Colony area was determined with ImageJ software. The values presented are mean ± SD from three independent experiments (n = 2 technical replicates). Unpaired Student’s t-test test was applied, ***p < 0.0001 calculated versus control (DMEM)