Fig. 4.

Upstream autophagic proteins in young and age, male and female mice. A. Representative western blots for Beclin1 and ATG7. B Quantification of Beclin1 protein in combined and sex-separated groups. C Quantification of ATG7 protein in combined and sex-separated groups. All values were corrected to GAPDH and are reported as means ± SEM, in A.U. The main effects of 2-way ANOVA are represented on a graph at p < 0.05. δ p < 0.05, post hoc significance. *p < 0.05, t-test between indicated groups. N = 10/male group, 8/female group