Fig. 6.

Mitophagy protein content in the muscle and mitochondria in young and aged, male and female mice. A Representative western blots for BNIP3 and Parkin in whole muscle samples. B Quantification of BNIP3 protein in combined and sex-separated groups. C Quantification of Parkin protein in combined and sex-separated groups. Whole muscle values were corrected to GAPDH. Values are reported as means ± SEM, in A.U. The main effects of 2-way ANOVA are represented on a graph at p < 0.05. δ p < 0.05, post hoc significance. *p < 0.05, t-test between indicated groups. N = 10/male group, 8/female group