Fig. 8.

Regulation of lysosome biosynthetic pathways in young and aged, male and female mouse muscle with exercise. A Representative western blots for TFEB and TFE3 protein. B Quantification of TFEB protein in combined and sex-separated groups. C Quantification of TFE3 protein in combined and sex-separated groups. D Representative western blots for TFEB protein in nuclear and cytosolic fractions in sedentary and exercised, young and aged, male and female mice. E % nuclear TFEB protein in combined and sex-separated male and female mice. F Fold-change in nuclear TFEB protein in each group examined. G TFEB promoter activity (luciferase; RLU) in young and aged, sedentary, and exercised mice. Values in B and C were corrected to GAPDH and are reported as means ± SEM. N = 10/male group, 8/female group. Line break in representative blot is different sections from the same blot. In D–F, cytosolic values were corrected to α-tubulin and nuclear values were corrected to H2B and reported as mean ± SEM, in A.U. N = 5/male group, 4/female group. In G, N = 6/group. The main effects of 2-way ANOVA are represented on graph at p < 0.05. δ p < 0.05, post hoc significance. *p < 0.05, t-test between indicated groups