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. 2022 Jun 11;41:198. doi: 10.1186/s13046-022-02412-3

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© The Author(s) 2022

Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data.

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Fig. 1 — NCAPD3 was higher expression in human CRC. A Expressions of NCAPD3 in CRC tissues and corresponding non-tumor normal tissues of clinic specimen by analyzing our RNA-seq data. B The levels of NCAPD3 mRNA in CRC tissues and normal tissues were analyzed by using GEPIA (left) and Assistant for Clinical Bioinformatics (right) web tools. G1, G2, G3 represent different tumor grades. C The level of NCAPD3 at different tumor stages were conducted by using online database. D Protein levels of NCAPD3 in human normal colorectal mucosal cell line FHC, CRC cell lines (RKO, HCT116 and SW480) and clinic specimen (including CRC tissues and normal colorectal tissues) were detected by Western blot assay. E qRT-PCR analysis of mRNA expression in 59 human CRC tissues and 13 normal colorectal tissues. F Immunohistochemistry (IHC) detection of NCAPD3 was carried out in CRC tissues and corresponding non-tumor normal colorectal tissues. Representative image of NCAPD3 staining were shown here. Scale bars: 100X = 400 μm; 400X = 100 μm. N represents Normal, T represents Tumor. Repetitions: n = 3 in A, D, F. Results were shown as mean ± s.d., *P < 0.05, **P < 0.01, ***P < 0.001, based on Student’s t test