Table 1. Genetic diversity indices and neutrality tests for each clade of Bufo japonicus and B. torrenticola based on cytochrome b sequences.

		N	N a	Hd ± SD	π ± SD	Fu’s FS
						F S	p-value
Bufo japonicus	clade A1	13	12	0.99 ± 0.04	0.006 ± 0.003	−5.46*	0.0061
	clade A2	33	29	0.99 ± 0.01	0.006 ± 0.003	−24.10*	0
	clade A3	83	57	0.98 ± 0.01	0.007 ± 0.004	−24.83*	0
	clade B1	45	36	0.99 ± 0.01	0.008 ± 0.004	−23.06*	0
	clade B2	28	26	0.99 ± 0.01	0.005 ± 0.003	−24.34*	0
Bufo torrenticola		25	19	0.97 ± 0.02	0.006 ± 0.003	−8.15*	0.0018

Note:

N, number of individuals; N_a, number of haplotypes; H_d, haplotype diversity; π, nucleotide diversity; SD, standard deviation. Asterisks indicate significant p-values (p < 0.01).