TABLE 2.

Closest relatives of bacterial phylotypes from O1 16S rRNA gene library

Division (% representation)	Type sequence	No. of RFLP typesa	No. of total clonesb	Closest cultivated species	Source	% Similarityc
Proteobacteria
γ (35%)	O1 1E2	1	1	Marinobacter hydrocarbonoclasticus	Seawater	99.4
	O1 6G1	3	11	Pseudomonas fluorescens	Soil	99.3–99.6
	O1 6A8	1	2	Pseudomonas putida	Subsurface	99.6
	O1 8F4	1	1	Pseudomonas aureofaciens	Soil	99.1
	O1 4B5	5	20	Halomonas variabilis	Salt lake	98.5–99.6
	O1 1D12	1	1	Halomonas pacifica	Seawater	97.2
	O1 8C4	3	7	Acinetobacter johnsonii	Sewage	99.5–99.9
	O1 1G12	1	2	Acinetobacter junii	Urine	100
	O1 8E12	1	4	Acinetobacter sp.	Subsurface	98.9–99.3
α (3%)	O1 4D1	1	1	Sphingomonas yanoikuyae	Soil	98.9
	O1 4C5	1	2	Brevundimonas diminuta	Soil	99.9
	O1 8E10	1	1	Methylobacterium radiotolerans	Lake water	100
β (0.6%)	O1 1E3	1	1	Not cultivated
δ (1.2%)	O1 1H2	1	2	Desulfomicrobium apsheronum	Oil	91.6
Flavobacterium, Bacteroides, and Cytophaga (0.6%)	O1 1D1	1	1	Flexibacter aurantiacus	Soil	97.6
Low G+C, gram positive (42%)	O1 8G7	3	40	Acidaminococcus fermentans	Rumen	94.7
	O1 1B1	13	18	Thermoanaerobacter brockii	Oil	95.8
	O1 1D9	1	1	Aminobacterium colombiense	Sewage	87.1
	O1 8G5	1	2	Desulfothiovibrio peptidovorans	Oil	93.7

^aNumber of unique RFLP patterns most closely related to the same cultivated species. 

^bNumber of total related clones in SSU O1 library. 

^c16S rDNA E. coli numbering 519 to 1,390.