Fig. 2.

Efficacy of the adoptive transfer of engineered B cells in HIS mice. Four cohorts of NSG mice were humanized with 4 different batches of huCD34⁺ cells. B cells were isolated from donor mice and injected into autologous HIS recipients after lentiviral transduction (Cohort #A (n = 5), #B (n = 9), #C (n = 2), #D (n = 11)). Five different LV batches were used for B cell transduction: LV #1 (n = 5), LV #2 (n = 7), LV #3 (n = 2), LV #4 (n = 2), LV #5 (n = 11). The control group was performed with non-transduced B cells (n = 7). (a) Gating strategy. Representative plots are presented. (b) Adoptive transfer (AT) efficacy calculated as the ratio of the numbers of infused GFP⁺ B cells to the numbers of GFP⁺ splenic B cells post-transfer. (c) Frequencies of huCD19⁺GFP⁺ cells among huCD45⁺ splenocytes in recipient HIS mice analyzed by flow cytometry 7 days after B cell transfer. (d) Absolute numbers of huCD19⁺GFP⁺ B cells in recipient’s spleens