Fig. 4.

Colorectal tumour conditioned media drives IL10 secretion. a Significantly higher level of IL-10 was detected by ELISA in the supernatant of CD14+HLA-DR- cells polarized by TCM (blood samples n=4, tumour tissue n=3) compared healthy control. b IL-10 receptor blocking antibody (10μg/ml) inhibits the suppressive activity of TCMs polarized CD14+HLA-DR- cells restoring T cell proliferation (n=4). Higher concentration of TGF-β was measured by ELISA in TCM (n=19) c and in the plasma d of colorectal patients (n=39 and healthy plasma n=10). e A representative histogram of HLA-DR expression on monocytes cultured with cytokines for 48h. f Down regulation of HLA-DR on monocytes cultured with cytokines for 48h was observed by Flow analysis (n=23 TGFβ, n=26 IL-6, n=11 IL-10, n=6 G-CSF, n=5 GM-CSF and n=4 VEGF)