Fig. 5.

TGF-β derived M-MDSC suppress T cell proliferation. a TGF-β polarized CD14+HLA-DRcells suppress T-cell proliferation. Less suppressive activity has shown from TGF-β polarized CD14+HLA-DR+ cells. The 1:0.5 ratio of T cells: myeloid cells shown (n=13). b High expression of p-SMAD and p-STAT3 was detected by Western blot in the CD14+HLA-DR- cells polarized by TGF-β.Representative of 4 experiments. c and d TGF-β receptor inhibitor inhibits the ability of TGF-β and TCMs to down regulate HLA-DR on polarized monocytes (n=4). e TGF- β receptor inhibitor inhibits the release of IL-10 from TGF-β and TCM polarized monocytes. Representative of 2 experiments. fTGF- β receptor inhibitor inhibits the suppressive activity of CD14+HLA-DR- cells polarized with TGF-β and CRC cell TCM (n=3)