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. 2022 Jun 11;8:284. doi: 10.1038/s41420-022-01071-9

Fig. 3. Caspase-2-mediated apoptotic signaling upon GO-treatment of AML cells does not involve conformational change of Bax.

Fig. 3

A Bax conformational change was studied in HL60 cells by flow cytometry after 48 h treatment with GO, calicheamicin or etoposide alone or combined with the caspase-2 inhibitor z-VDVAD-fmk (10 µM, 2 h pre-incubation). Left panel: Bax conformational change is shown as a peak-shift to the right in the flow cytometry histogram. Right panel: Quantification of Bax-associated mean fluorescence intensity. Data is given as fold increase to untreated cells. B Cleavage of full-length Bid was analyzed by immunoblotting at 24 h post treatment with indicated doses of GO with or without 2 h pre-incubation with z-VDVAD-fmk (10 µM). GAPDH was used as loading control.