Fig. 2. SPPL3^KO suppresses CD19 CAR T cell activation.

a Schematic of T cell activation studies. b–d, Expression of b CD69, c PD1, and d Tim3 on T cells after overnight co-culture with WT or SPPL3^KO Nalm6 cells or alone (control). e expression of CD107a on T cells after a 4-h co-culture with WT or SPPL3^KO Nalm6 cells. f, g Expression of f GFP and g eCFP, reflecting binding of NFAT and NFκB to their respective promoter sites in CD19 CAR-expressing Jurkat cells. h Schematic of long-term co-culture and re-challenge study. i Survival of WT Nalm6 over time after the combination of CART19 cells previously cultured with either WT or SPPL3^KO Nalm6 cells. b–e, i Representative of n = 2 individual experiments with distinct donor T cells. f, g representative of n = 4 individual experiments. Error bars reflect mean ± standard error of the mean (s.e.m.). ****P < 0.0001 by two-way ANOVA with Bonferroni correction for multiple comparisons. Source Data are provided as a Source Data file.