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. Author manuscript; available in PMC: 2022 Jun 12.
Published in final edited form as: J Invest Dermatol. 2019 Aug 29;140(3):645–655.e6. doi: 10.1016/j.jid.2019.08.436

Figure 1: LL-37 enables UVB and dsRNA to increase adhesion molecule expression on HDMECs.

Figure 1:

(a) Facial skin from healthy and rosacea subjects were stained VCAM1, ICAM1, E-Selectin and LL-37 antibody (green) and nuclear stain DAPI (blue). E=Epidermis and D=Dermis, Scale= 20 μM (b) UVB exposed and non-irradiated NHEKs extract were added onto HDMECs (12 h). Gene expression of adhesion molecules VCAM1, ICAM1 and SELE was assessed with q-RT PCR. (n=3) (c) UVB NHEKs were treated with RNAse III, then extracts co-cultured with HDMECs and mRNA expression of VCAM1, ICAM1 and SELE was assessed. (n=3) (d, e, f) Secreted adhesion molecules was analyzed by ELISA. (n=3). (g, h) Immunoblot (g) and immunofluorescence (h) analysis for adhesion molecules. Scale = 20 μM. (*, p< 0.05; **, p<0.01; ***, p<0.001; ****, p<0.0001)