Skip to main content
. Author manuscript; available in PMC: 2022 Jun 12.
Published in final edited form as: Cancer Biomark. 2015;15(3):281–287. doi: 10.3233/CBM-150469

Table 2.

Results of purification of anti-Pg IgG at each stage. IgG amount is the mean of four independent experiments with the same plasma pool

Stage of
purificationPlasma samples, V = 1 ml
Intact plasma,
total IgG, mg
Ammonium sulfate
precipitation.
Total IgG, mg
Protein-G Sepharose,
total IgG, mg
IgG, bound to Pg-
sepharose, mg
IgG, unbound to Pg-
sepharose, mg
Plasma of healthy donors 9.0 8.1 ± 0.4 7.5 ± 0.6 0.7 ± 0.2 6.0 ± 0.5
Plasma of prostate cancer patients 12.3 11.0 ± 0.6 10.3 ± 0.9 2.8 ± 0.4 6.5 ± 0.6