. Author manuscript; available in PMC: 2022 Jun 12.

Published in final edited form as: J Phys Chem B. 2019 Jul 1;123(27):5690–5699. doi: 10.1021/acs.jpcb.9b02443

Table 1.

Composition of the samples and the results of the Williamson-Hall plot analysis, including ice crystallite size, D, and microstrain, ε.

Sample composition		Ih crystal properties
Protein type & concentration	Buffer and other solutes	D, Å	Strain×10³
mAb 100 mg/ml	20 mM Histidine, pH 5.8	55±6 51±7^*	20±8 21±9^*
mAb 100 mg/ml	20 mM Histidine, pH 5.8, 5 % w/v sucrose, 0.02 %w/v PS80	66±14	21±10
rHA 100 mg/ml	20 mM Histidine, pH 5.8	60±8	22±8
lysozyme 100 mg/ml	20 mM Histidine, pH 5.8	108±11 94±8^*	11±4 8±4^*
iAFP 1 mg/ml	Water	89±6 78±4^*	9±4 9±4^*
iAFP 1 mg/ml	20 mM Histidine, pH 5.8	105±6	13±3
iAFP 0.5 mg/ml	Water	104±5	11±3
-	5 %w/v sucrose	104±3	6±2
-	10 %w/v sucrose	128±7	7±2
-	Water	119±5 77±4^*	7±2 7±4^*
-	20 mM histidine buffer, pH 5.8	78±2	6±3

Values determined from the replicate experiment