Figure 2. Enzyme activity of PLA2s toward sn-1 alkyl ether phospholipids and diester phospholipids.
The activity of (A) GIVA cPLA2, (B) GVIA iPLA2, and (C) GV sPLA2 is shown toward sn-1 alkyl ether or ester 16:0 sn-2 X PC, where X is 2:0, 18:1, or 20:4. (D) The activity of GIVA cPLA2 toward sn-1 alkyl ether or ester 16:0 sn-2 20:4 PC in equal proportions with 100 µM total phospholipid. The activity of (E) GVIA iPLA2 and (F) GV sPLA2 toward sn-1 alkyl ether or ester 16:0 sn-2 18:1 PC in equal proportions with 100 µM total phospholipid. The amount of lyso products were determined and quantified using authentic LPC primary and internal standards. Error bars show standard deviation (SD). N.D.: not detected. Asterisks indicate statistical significance. **P < 0.01.