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. 2022 Jun 13;40(1):111022. doi: 10.1016/j.celrep.2022.111022

Figure 3.

Figure 3

Cross-reactive antibodies dominate the responses to FP and SH across time, with the emergence of late monoreactivity to SARS-CoV-2 FP

(A) To quantify the extent of cross-reactivity between SARS-CoV-2 and HCoV-OC43 at the two conserved Spike epitopes, vaccinee plasma samples were treated with beads bearing biotinylated FP + SH peptide pools of either the SARS-CoV-2 (orange) or the HCoV-OC43 (blue) sequences to deplete binding antibodies. The resulting samples were assayed by PepSeq, wherein a reduction in signal corresponding to the non-depleting species would indicate the presence of cross-reactive IgG antibodies.

(B) Change in PepSeq signal for the SARS-CoV-2 and/or HCoV-OC43 homologs of the SD1 (SARS-CoV-2 only, top), FP (middle), or SH (bottom) epitopes at the indicated post-vaccination time point, after depletion by FP + SH peptide pools of either the SARS-CoV-2 (orange) or the HcoV-OC43 (blue) sequences. The limits of the boxes correspond to the first and third quartiles, the black lines inside each box correspond to the median, and the whiskers extend to points that lie within 1.5 interquartile ranges of the first and third quartiles. Plots include only subject:time point combinations with detectable reactivity for either SARS-CoV-2 or HCoV-OC43 at the focal epitope (Z > 10). Each point represents the mean of two technical replicates. Number of samples included in each boxplot range from 1 to 10 as indicated; t test: ∗∗p < 0.01, ∗∗∗p < 0.001.