TABLE 3.
Accumulation of poly(3HB) by recombinant strains of E. coli JM109 harboring different plasmidsa
| Medium | Poly(3HB) content (% of cell dry wt)
|
||
|---|---|---|---|
| JM109 (pJC7) | JM109 (pBPP1) | JM109 (pBPha) | |
| M9 | 0.2 | 0.4 | 0.3 |
| LB | 0.2 | 0.2 | 0.3 |
| M9 + glucose (1%) | 0.3 | 0.3 | 0.3 |
| LB + glucose (1%) | 0.3 | 0.3 | 0.3 |
| M9 + glucose (1%) + 3HB (0.4%) | 0.4 | 43.3 | 0.5 |
| LB + glucose (1%) + 3HB (0.4%) | 0.4 | 5.1 | 0.4 |
a
Strains were cultivated in 300-ml Erlenmeyer flasks containing 50 ml of medium with the indicated carbon source, 1 mM isopropyl-β-d-thiogalactopyranoside and 100 mg of ampicillin per liter at 37°C and at 200 rpm for 72 h.