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| Other reagents | Final concentration |
|---|---|
| 5% NaHCO3 | 2.5 g NaHCO3 in 50 mL ddH2O |
| 2% Oxoid agar | 10 g Oxoid agar in 500 mL ddH2O |
| Blocking solution | 1× TBST and 5% non-fat dry milk |
| Antibody dilution buffer | 1× TBST and 3% non-fat dry milk |
Keep at RT. Always prepare and use fresh.
