PCR cycling conditions
| Steps | Temperature | Time | Cycles |
|---|---|---|---|
| Reverse Transcription (RT) | 50°C | 20 min | 1 |
| RT heat inactivation | 95°C | 1 s | 1 |
| DNA polymerase activation | 95°C | 5 min | 1 |
| Denaturalization | 95°C | 15 s | 40 |
| Annealing/Extension | 60°C | 45 s | |
| Hold | 4°C | Forever | |
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