Lysis buffer
| Reagent | Final concentration | Amount |
|---|---|---|
| RIPA buffer | 1× | 10 mL |
| EDTA-free protease inhibitor cocktail (tablets) | N/A | 1 tablet |
| Total | N/A | 10 mL |
Reagents are kept at 4°C. Always prepare and use fresh.
Lysis buffer
| Reagent | Final concentration | Amount |
|---|---|---|
| RIPA buffer | 1× | 10 mL |
| EDTA-free protease inhibitor cocktail (tablets) | N/A | 1 tablet |
| Total | N/A | 10 mL |
Reagents are kept at 4°C. Always prepare and use fresh.