| Cycle no. | Denature | Anneal | Extend |
|---|---|---|---|
| 1 | 72 °C, 5 min | ||
| 2 | 98 °C, 30 s | ||
| 3-5 (3 cycles) | 98 °C, 10 s | 65 °C, 30 s | 65 °C, 45 s |
| Hold at 4 °C |
Official websites use .gov
A
.gov website belongs to an official
government organization in the United States.
Secure .gov websites use HTTPS
A lock (
) or https:// means you've safely
connected to the .gov website. Share sensitive
information only on official, secure websites.