FIG. 3.
Northern blot analysis of ATF1 transcripts prepared from yeast cells during fermentation. RNA from commercial wine yeast strains VIN7, VIN13, and WE228 together with their corresponding transformants carrying the constitutively expressed PGK1P-ATF1-PGK1T gene cassette, VIN7(pATF1-s), VIN13(pATF1-s), and WE228(pATF1-s), was isolated on days 3, 5, 7, 9, and 11, subjected to formamide gel electrophoresis, and probed for ATF1 and ACT1. The sizes of the ATF1 and ACT1 transcripts were determined relative to a Bio-Rad Laboratories RNA molecular size marker.