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. 2022 May 30;15:100307. doi: 10.1016/j.mtbio.2022.100307

Fig. 5.

Fig. 5

In vitro evaluation of r-BMSCs osteogenic differentiation on/in the microgels. (A) Schematic illustration of cell osteogenic differentiation on/in the microgels after 4 and 7 days of osteogenic incubation. (B) The protein expression of osteogenic markers of r-BMSCs. (C) Quantitative analysis of ALP, OCN, and COL-1 protein expression for 4 and 7 days on/in microgels, normalized to GAPDH. (D) qRT-PCR analysis of ALP, OCN, and COL-1 gene expression of r-BMSCs during 4 and 7 days of osteogenic incubation respectively. (E) OCN staining of microgels at day 4, 7 post-osteogenic incubation (red arrowheads: positive brown staining, scale bars: 100 ​μm, ns: no significance, ∗∗P ​< ​0.01, ∗∗∗P ​< ​0.001).