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. 2022 May 30;10:800574. doi: 10.3389/fcell.2022.800574

FIGURE 5.

FIGURE 5

GSK′872 and N-acetyl cysteine equally suppress RIPK3 expressions in high glucose-treated renal podocytes. A histogram shows RIPK3 expression in HG, GSK′872, or antioxidant-treated podocytes. Subsequent to 24-h starvation using serum-free media, each cell plate of podocytes was incubated with HG (30 mM), GSK′872 (RIPK3 inhibitor, 10 μM), or an antioxidant (NAC, 50 μM) for another 24 h. Plates were then suctioned and washed three times with PBS. Podocytes were harvested by adding 90 μL of PRO-PREP™ solution to each cell plate to perform Western blot analysis. The expression of RIPK3 was significantly lower in both GSK′872-treated and NAC-treated cell plates, which did not show any statistically significant difference from the negative control group. NG, normal glucose; HG, high glucose; GSK, GSK′872; NAC, N-acetyl cysteine. ***, p < 0.001 versus NG; +++, p < 0.001 versus HG.