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. 2022 May 30;15:916458. doi: 10.3389/fnmol.2022.916458

FIGURE 2.

FIGURE 2

Sal reduces cisplatin-induced apoptosis in HEI-OC1 cells. (A) Cleaved caspase 3 (red) and DAPI (blue) double staining showing apoptotic cells after the different treatments (n = 3). (B) Quantitative analysis of apoptotic cells in (A). (C) Western blotting results showing cleaved PARP (n = 3) and cleaved caspase 3 (n = 3) in the different groups. (D,E) Quantitative analysis of the western blotting results in (C). Values are presented as mean ± SEM from independent experiments. *P < 0.05 and ***P < 0.001 vs. the control group; &P < 0.05, &&P < 0.01, and &&&P < 0.001 vs. the cisplatin group. Sal, salubrinal; Cis, cisplatin. Scale bars = 20 μm.