FIGURE 3.

Sal promotes hair cell (HC) survival in the cochlea after cisplatin exposure. (A) Schematic diagram of drug addiction in tissue culture. (B,D) HCs in the apical (B), middle (C), and basal turns (D) of the cochlea were stained with anti-myosin-VIIa antibody and DAPI in the control, 10 μM Sal, 20 μM cisplatin, and 20 μM cisplatin + 10 μM Sal groups. (E) Quantification of the number of myosin VIIa-positive cells in the apical, middle, and basal turns of the cochlea. Values are presented as mean ± SEM from independent experiments. *P < 0.05 and ***P < 0.001 vs. the control group; ^&P < 0.05 and ^&&&P < 0.001 vs. the cisplatin group. Scale bars = 10 μm.