TABLE 1.
Sequences of primers used for real‐time quantitative polymerase chain reaction (RT‐qPCR) in this study
| Gene | Primer sequence (forward) | Primer sequence (reverse) |
|---|---|---|
| GAPDH | AGGTCGGTGTGAACGGATTTG | TGTAGACCATGTAGTTGAGGTCA |
| TLR4 | ATGGCATGGCTTACACCACC | GAGGCCAATTTTGTCTCCACA |
| NLRP3 | ATTACCCGCCCGAGAAAGG | TCGCAGCAAAGATCCACACAG |
| GSDMD | CCATCGGCCTTTGAGAAAGTG | ACACATGAATAACGGGGTTTCC |
| ASC | CTTGTCAGGGGATGAACTCAAAA | GCCATACGACTCCAGATAGTAGC |
| Caspase‐1 | TGGAAATGTGCCATCTTCTTT | TCAGCTCCATCAGCTGAAAC |
| IL‐1β | CGAAGACTACAGTTCTGCCATT | GACGTTTCAGAGGTTCTCAGAG |
| IL‐18 | ACTTCACTGTACAACCGCA | TCAGTCATATCCTCGAACAC |
| DDX3X | GGATCACGGGGTGATTCAAGAGG | CTATCTCCACGGCCACCAATGC |
| BGN | GACAACCGTATCCGCAAAGT | CAAAGGCTCCTGGTTCAAAG |
Abbreviations: ASC, associated speck‐like protein containing a caspase recruitment domain; BGN, biglycan; DDX3X, dead‐box helicase 3 X‐linked; GAPDH, glyceraldehyde 3‐phosphate dehydrogenase; IL, interleukin; NLRP3, NLR family pyrin domain containing 3; TLR4, Toll‐like receptor 4.