TABLE 2.
A. niger strain | No. of copies of pAS | Specific refolding activity at 44 h (U/mg) | Level of glucoamylase at 72 h (mg/liter) |
---|---|---|---|
AB4.1 | 0 | 1.4 ± 0.1 | 166 ± 7 |
AS1.1 | 6–10 | 0.7 ± 0.1 | 58 ± 6 |
AS2.2 | 4–6 | 1.0 ± 0.1 | 65 ± 6 |
Refolding activities are presented in units per milligram of microsomal protein. Levels of glucoamylase secreted were assayed from the supernatant of 72-h cultures grown in ACMS/N/P. Values represent the average ± the SEM from triplicate experiments.