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. 2022 Jun 10;20(2):15593258221105704. doi: 10.1177/15593258221105704

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© The Author(s) 2022

This article is distributed under the terms of the Creative Commons Attribution-NonCommercial 4.0 License (https://creativecommons.org/licenses/by-nc/4.0/) which permits non-commercial use, reproduction and distribution of the work without further permission provided the original work is attributed as specified on the SAGE and Open Access pages (https://us.sagepub.com/en-us/nam/open-access-at-sage).

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Figure 2. — Methylation status of actinin alpha 4 (ACTN4) in HPC cells and ACTN4 gene demethylation induced by EGCG. M: Methylation-specific PCR. U: Unmethylation-specific PCR. (A): Methylation status of ACTN4 in HPC cells. Line 1: Methylation status of ACTN4 in HPC without HG treatment. Line 2: Methylation status of ACTN4 in the diabetic nephropathy (DN) cell model. Line 3: Unmethylated positive control. Line 4: Methylated positive control. Line 5: Negative Control. (B): ACTN4 demethylation induced by EGCG.