Figure 5.

Lysosomal Zn²⁺ release in various cell types measured by GZnP3

(A–D) ML1-mediated Zn²⁺ signals measured by GZnP3 responses in (A) primary rat hippocampal neurons, (B) INS-1 cells, (C) COS-7 cells, and (D) HeLa cells expressing GZnP3 and mCherry-ML1. Cells were treated with 50 μM ML-SA1 at 0 s and 100 μM TPEN at 300 s.

(E) Bar graph of maximum GZnP3 signal (ΔF/F₀) for each of the four cell types. Mean GZnP3 signal maximum (±SEM) 300 s after ML-SA1 addition. (Figure reprinted with permission from Minckley et al. [2019].)

(F) In situ response of GZnP3 to Zn²⁺ chelation and saturation. Representative GZnP3 signals in HeLa cells treated with 100 μM TPEN at 0 s, washed three times with Zn²⁺, Ca²⁺ free HBSS, then treated with 100 μM ZnCl₂ and 2.5 μM pyrithione at 300 s.