Tyrode’s solution
| Reagent | Final concentration (mM) | Amount |
|---|---|---|
| NaCl | 145 | 8.474 g |
| Glucose | 10 | 1.802 g |
| HEPES | 20 | 4.766 g |
| CaCl2 | 2 | 0.3803 g |
| MgCl2 | 1 | 0.406 g |
| KCl | 5 | 0.3728 g |
| ZnCl2 | 0.1 | 0.0136 g |
| ddH2O | n/a | To 1 L |
| Total | n/a | 1 L |
Adjust the pH to 7.4 with 10 N NaOH.
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