REAGENT or RESOURCE	SOURCE	IDENTIFIER
Chemicals, peptides, and recombinant proteins
DMEM high glucose medium	Thermo Fisher Scientific	Cat#11965092
Phosphate Buffered Saline (PBS)	Thermo Fisher Scientific	Cat#20012027
Poly-D-Lysine (PDL)	Gibco	Cat#A3890401
Opti-MEM	Gibco	Cat#31985070
Lipofectamine 2000	Thermo Fisher Scientific	Cat#11668019
Sodium chloride, NaCl	Sigma-Aldrich	S9888; CAS: 7647-14-5
Calcium chloride, CaCl2	Sigma-Aldrich	C8106; CAS: 10035-04-8
Magnesium chloride, MgCl2	Sigma-Aldrich	208337; CAS: 7786-30-3
Potassium chloride, KCl	Sigma-Aldrich	P9541; CAS: 7447-40-7
Zinc chloride, ZnCl2	Sigma-Aldrich	208086; CAS: 7646-85-7
Glucose	Sigma-Aldrich	G6152; CAS: 50-99-7
HEPES	Sigma-Aldrich	H3375; CAS: 7365-45-9
Glycyl-L-phenylalanine 2-naphthylamide (GPN)	Santa Cruz	sc-252858; CAS: 21438-66-4
ML-SA1	Tocris	4746; CAS: 332382-54-4
Pyrithione	Cayman	29154; CAS: 13463-41-7
TPEN	Cayman	13340; CAS: 16858-02-9
Experimental models: Cell lines
INS-1	Sigma-Aldrich	SCC207
COS-7	ATCC	ATCC CRL-1651
HeLa	ATCC	ATCC CCL-2
MeWo	ATCC	ATCC HTB-65
Recombinant DNA
GZnP3	Addgene	Plasmid #161738
GZnP3-ML1	Minckley et al. (2019)	N/A
mCherry-ML1	Du et al. (2021)	N/A
Software and algorithms
EasyRatioPro	HORIBA	https://www.horiba.com/
OriginPro	OriginLab	https://www.originlab.com/
Other
18 mm Ø Cover glasses circular, #1.5	Thermo Fisher Scientific	Cat#50-948-975
12-well Clear TC-treated Multiple Well Plates	Corning	3513
0.2 μm pore size sterile syringe filter	Corning	431229
Metal perfusion chamber	Du et al. (2021)	N/A
35 mm imaging dish	MatTek	P35GC-1.5-14-C