Figure 4.

Example spatial localization of CD4⁺ T cell clones

(A) H&E staining of a lung cancer metastasis to the brain.

(B) Clusters of gene expression within the tissue.

(C) scRNA-seq was performed on CD4⁺ T cells from three patients. Cells are plotted based on their UMAP dimensionality reduction coordinates and colored by gene expression cluster.

(D) Expression of selected genes.

(E and F) Spatial localization of a granzyme-expressing CD4⁺ T cell clone in (E) and multiple Treg clones in (F).

(G and H) scRNA-seq phenotype of these clones are shown in panels (G and H).