REAGENT or RESOURCE	SOURCE	IDENTIFIER
Biological samples
Amplified cDNA from step 3.4 of the Visium Spatial Gene Expression Reagent Kits User Guide	User	n/a
Chemicals, peptides, and recombinant proteins
Nuclease-free water	Thermo Fisher Scientific/Ambion	Cat# AM9932
Library Construction Kit	10x Genomics	Cat# PN-1000190
SPRIselect beads	Beckman Coulter	Cat# B23317
Buffer EB	QIAGEN	Cat# 19086
Critical commercial assays
Bioanalyzer with high sensitivity DNA kit and reagents	Agilent	Cat# 5067-4626
Qubit instrument with dsDNA HS Assay kit (optional)	Thermo Fisher Scientific	Cat# Q32854
Oligonucleotides
TCRB + read 1 primers and read 2 primer (see Table S1 for sequences)	Integrated DNA Technologies	n/a
Dual Index Kit TT Set A sample index primers	10x Genomics	Cat# PN-1000215
Software and algorithms
MiXCR (Bolotin et al., 2015)	GitHub or Homebrew	https://github.com/milaboratory/mixcr or https://mixcr.readthedocs.io/en/master/install.html
Example spatial TCR sequencing code	This paper and Sudmeier et al., Cell Reports Medicine 2022	https://github.com/whhudson/spatialTCR (https://doi.org/10.5281/zenodo.6368907)
Deposited data
scRNA-seq data	Sudmeier et al., Cell Reports Medicine 2022	GEO: GSE179373
Spatial transcriptomics data	Sudmeier et al., Cell Reports Medicine 2022	GEO: GSE179572
Spatial TCR-sequencing reads	Sudmeier et al., Cell Reports Medicine 2022	BioProject: PRJNA742564
Other
Magnetic Separator for PCR tubes	10x Genomics	Cat# 120250
Thermocycler (e.g., Nexus GX2)	Eppendorf	Cat# 6336000023
Widefield color brightfield microscope (e.g., Lionheart FX)	BioTek	n/a
Sequencer (e.g., Illumina MiSeq)	Illumina	n/a