Figure 5.

Overview of the batch effects and systematic bias of the assay method.

(A and C) The heat map of hierarchical clustering analysis (A) and scatter plot of t-SNE analysis (C) of the MRM datasets in terms of cohorts. Different cohorts were marked by colors (green, training cohort; red, test cohort; blue, validation cohort). (B and D) The heat map of hierarchical clustering analysis (B) and scatter plot of t-SNE analysis (D) of the MRM datasets in terms of medical centers. Samples collected at different medical centers were marked by colors (red, malignant brain gliomas samples from Tiantan hospital (MBGs); green, healthy control samples from Haidian hospital (NC1); blue, healthy control samples from Peking University Third Hospital (NC2); light blue, healthy control samples from Tiantan Hospital (NC3)).