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. 2022 Jun 10;10(6):e004268. doi: 10.1136/jitc-2021-004268

Figure 2.

Figure 2

TL8-506-based combinations synergize in the production of cytokines and chemokines in human blood DC subsets. Human peripheral blood mononuclear cells (PBMCs) were treated with the indicated stimuli for three hours. Intracellular cytokine staining was performed and analyzed by flow cytometry. (A) Quantification of cytokine producing blood cDCs, n=two–11 donors, from two to 16 independent experiments. (B) Flow cytometry plots of IFN-γ+TL8-506 and single stimuli treated blood cDCs showing the distribution of CXCL10 or/and TNF-α expressing cDCs. Percentage of positive cells are indicated in the quadrants. (C) Quantification of CXCL10/TNF-α double positive blood cDCs on stimulation with the indicated stimuli, n=four–eight donors, from four to eight independent experiments. (D) Flow cytometry plots of Poly(I:C)+TL8-506 and single stimuli treated blood cDCs showing the distribution of IFN-λ or/and TNF-α expressing cDCs. Percentage of positive cells are indicated in the quadrants. (E) Quantification of IFN-λ/TNF-α double positive blood cDCs on stimulation with the indicated stimuli, n=four–six donors, from four to six independent experiments. Statistics for A, C, E: paired Student’s t-test, *p≤0.03, ****p≤0.0001, ns, not significant. The following concentrations were used for DC stimulation: 50'000 U/mL huIFN-γ, 10 µg/mL Poly(I:C), 1 µM TL8-506. cDC, conventional DC; DC, dendritic cell; IFN, interferon; TNF, tumor necrosis factor.