Figure 3.

DuoBody-CD40×4-1BB induces DC maturation. Immature DCs from healthy donors were cocultured with purified, allogeneic CD8⁺ T cells in the presence of DuoBody-CD40×4-1BB, Fc-inert analogs of clinical mAbs mitazalimab and urelumab (0.001–1 µg/mL) or control antibodies (1 µg/mL; for combination of control antibodies 1 µg/mL of both antibodies was added) for 5 days. The percentage of HLA-DR⁺CD86⁺ DCs was measured by flow cytometry. Data from one donor are shown. Dotted line shows percentage of HLA-DR⁺CD86⁺ DCs in DC-T-cell cultures in the absence of treatment. DC, dendritic cell.