Table 2. Target Inhibition by NBTIs^a.

compd	DNA gyrase IC50 (μM)b	TopoIV IC50 (μM)c	TopoIV/gyrase ratio
2a	0.047 (3)	0.54 (3)	11
3a	0.23 (2)	0.60 (2)	2.6
4a	0.23 (2)	1.7 (2)	7.6
2b	2.1 (2)	1.6 (2)	0.76
3b	0.58 (3)	2.9 (3)	5.0
2c	1.8 (3)	10 (2)	5.6
3c	1.7 (2)	7.4 (2)	4.4
3d	3.5 (2)	9.7 (2)	2.8
2e	4.2 (2)	1.4 (2)	0.33
3e	1.6 (2)	1.3 (5)	0.81
4e	0.19 (1)	1.1 (2)	5.8
2f	1.4 (2)	2.1 (2)	1.5
4f	0.15 (1)	0.42 (1)	2.8
gepotidacin (racemic)	0.17 (2)	2.8 (3)	16
ciprofloxacin	28 (2)	8.6 (2)	0.31

^aAverage IC₅₀ values from full concentration–response curves performed on separate days, with the number of replicate experiments in parentheses.

^bInhibition of supercoiling activity.

^cInhibition of decatenation activity.