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. 2022 May 30;13:867302. doi: 10.3389/fimmu.2022.867302

Figure 1.

Figure 1

Notch signaling is active in vivo in human and mouse postnatal TECs. Immunohistochemistry of the canonical Notch target Hes1 (red) in postnatal human (≤ 6-years) and mouse (≤ 5-months) thymus. TECs are characterized by expression of pCK (green). Topro3 shows nuclear staining (blue). (A) General view (scale bar: 100μm) of Hes1 expression in a representative human thymus sample (18-months), and detailed view (scale bar: 50μm) of Hes1 and pCK expression in the thymus cortex and medulla. Dotted line, corticomedullary junction; c, cortex; m, medulla. Arrowheads indicate Hes1 expression in TECs (pCK+); h, Hassal’s corspuscles. (B) Bar graphs show numbers of Hes1+ nuclei within pCK+ ROIs relative to total (Topro+) cellular areas analyzed in the human thymus cortex and medulla. Data are shown as mean numbers ± SEM per field obtained from n= 8-10 different 63x images from sample, (n ≥ 2 independent human thymus samples aged ≤ 6-years). ****p < 0.0001. (C) General view (scale bar: 100μm) of Hes1 expression in a representative mouse thymus sample (5 months), and detailed view (scale bar: 50μm) of Hes1 and pCK expression in the thymus cortex and medulla. Dotted line, corticomedullary junction; c, cortex; m, medulla. Arrowheads indicate Hes1 expression in TECs (pCK+). (D) Bar graphs show numbers of Hes1+ nuclei within pCK+ ROIs relative to total (Topro+) cellular areas analyzed in the mouse thymus cortex and medulla. Data are shown as mean numbers ± SEM per field obtained from n= 10 different 63x images from sample, (n ≥ 2 independent mouse thymus samples aged ≤ 5-months). ****p < 0.0001.