Table 1.

Cryo-EM data collection, refinement, and validation statistics

Complex and State	dPlmCasX- sgRNAv1- dsDNA State I	dPlmCasX- sgRNAv1- dsDNA State II	dPlmCasX- sgRNAv1- dsDNA State III	dPlmCasX- sgRNAv2- dsDNA State I	dPlmCasX- sgRNAv2- dsDNA State II	dPlmCasX- sgRNAv2- dsDNA State III
EMDB code	EMD-32389	EMD-32390	EMD-32391	EMD-32392	N/A	N/A
PDB code	7WAY	7WAZ	7WB0	7WB1	N/A	N/A
Data collection and processing
Magnification	135,000	135,000	135,000	135,000	135,000	135,000
Voltage (kV)	300	300	300	300	300	300
Electron exposure (e–/Å2)	~50	~50	~50	~50	~50	~50
Defocus range (μm)	0.5~2.0	0.5~2.0	0.5~2.0	0.5~2.0	0.5~2.0	0.5~2.0
Pixel size (Å)	0.94	0.94	0.94	0.94	0.94	0.94
Symmetry imposed	C1	C1	C1	C1	C1	C1
Final particle images (no.)	520,115	502,778	710,824	616,493	267,147	143,849
Map resolution (Å)	2.9	3.4	3.2	3.7	N/A	N/A
FSC threshold	at 0.143	at 0.143	at 0.143	at 0.143
Map resolution range (Å)	2.5~6	3~7	3~7	3~7
Refinement
Model resolution (Å)	2.9	3.4	3.2	3.7
FSC threshold	0.143	0.143	0.143	0.143
Model resolution range (Å)	2.5~6	3~7	3~7	3~7
Map sharpening B factor (Å2)	−70	−120	−100	−137
Model composition	11453	11034	10096	11524
Non-hydrogen atoms
Protein residues	960	952	797	960
Nucleotides	175	157	175	178
B factors-Mean (Å2)
Protein	65.72	116.41	83.97	123.99
Nucleotide	106.17	161.06	157.90	157.80
R.m.s. deviations
Bond lengths (Å)	0.004	0.002	0.003	0.003
Bond angles (°)	0.542	0.589	0.579	0.590
Validation
MolProbity score	1.45	2.43	1.65	1.90
Clashscore	5.49	11.37	7	11.07
Poor rotamers (%)	0.00	5.46	0	0
Ramachandran plot
Favored (%)	97.17	95.69	96.57	95.18
Allowed (%)	2.83	4.10	3.3	4.72
Disallowed (%)	0.00	0.21	0.1	0.1