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. Author manuscript; available in PMC: 2022 Jun 13.
Published in final edited form as: Nat Struct Mol Biol. 2022 Apr 14;29(4):395–402. doi: 10.1038/s41594-022-00756-0

Fig. 1 |. Trapping the Cas9 interrogation complex.

Fig. 1 |

a, Known steps leading to Cas9-catalyzed DNA cleavage. Orange/black arrows indicate direction of guide RNA strand invasion into the DNA helix. Magenta X indicates location of cystamine modification. b, Chemistry of protein:DNA cross-link. c, RNA and DNA sequences used in structural studies. d, Sharpened cryo-EM map (threshold 7σ) and model of the cross-linked Cas9:sgRNA:DNA complex (0 RNA:DNA matches, bent DNA), centered on density contributed by the non-native thioalkane cross-link.