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. Author manuscript; available in PMC: 2022 Jun 13.
Published in final edited form as: Nat Struct Mol Biol. 2022 Apr 14;29(4):395–402. doi: 10.1038/s41594-022-00756-0

Fig. 6 |. Structure of a nascent R-loop and overview model.

Fig. 6 |

a, Left, unsharpened cryo-EM map (threshold 5σ) of Cas9:sgRNA:DNA with 3 RNA:DNA bp. Green, NUC lobe; blue, REC lobe; orange, guide RNA; magenta, DNA. Black vectors indicate differences from the bent-DNA structure with 0 RNA:DNA matches. The primary difference is a rigid-body rotation of REC lobe domain 2. Inset, sharpened cryo-EM map (threshold 8σ) and model. For clarity, only DNA and RNA are shown. Black, candidate complementarity region; yellow, PAM. b, Model for bend-dependent Cas9 target search and capture. Large diagrams depict states structurally characterized in the present work.