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. 2022 May 9;6(6):e10630. doi: 10.1002/jbm4.10630

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© 2022 The Authors. JBMR Plus published by Wiley Periodicals LLC on behalf of American Society for Bone and Mineral Research.

This is an open access article under the terms of the http://creativecommons.org/licenses/by/4.0/ License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited.

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Fig. 5 — Reduced osteoid fraction in the proximal P4ha1 ^+/−; P4ha2 ^−/− tibias at 6 weeks of age. (A) A Masson‐Goldner's trichrome‐stained trabecula in the proximal tibia showing mineralized ECM in green and unmineralized ECM in red (white arrowheads) at 6 weeks of age. (B–D) Quantification of osteoid volume/tissue volume (OV/TV) (B), osteoid surface (OS/BS) (C), and osteoid width (Os.Wi) (D) in the proximal tibia at 6 weeks and 6 months of age. The data in B–D are shown as box and whisker plots including all individual data points, median, and interquartile range (25th to 75th percentile). Statistical analysis was done with one‐way ANOVA followed by post hoc Dunnett's multiple comparisons test against the control P4ha2 ^+/− mice, n = 5–9 mice/genotype. Statistically significant p values are shown in the graphs.