Fig. 2 ∣. Biophysical characterization of UNC6934.

a, SPR analysis of the binding of UNC6934 to NSD2-PWWP1. Left, representative sensorgram (green) with the kinetic fit (black dots). Right, steady-state response (black circles) with the steady-state 1:1 binding model fitting (red dashed line). The indicated kinetic data were obtained from triplicate experiments (Extended Data Fig. 1). b, Selectivity profile of UNC6934 and UNC7145 against a panel of PWWP domains measured by DSF (compounds were tested at 100 μM in three independent experiments). c, Effect of UNC6934 and UNC7145 on the binding of NSD2-PWWP1 to nucleosomal H3K36me2, as measured by AlphaScreen. Each binding interaction was performed in triplicate (n = 3 independent experiments), and all data were normalized to the DMSO control. d,e, Effect of UNC6934 and UNC7145 on the binding of fl-NSD2 to nucleosomal H3K36me2 (n = 2 independent experiments), as measured by AlphaScreen, in the presence (d) or absence (e) of SSD (2 μg ml⁻¹).