Extended Data Fig. 2 ∣. Binding of UNC6934 and UNC7145 to wt-NSD2-PWWP1 and its F266A mutant.

The binding of both compounds to NSD2-PWWP1 F266A mutant in parallel to the wild-type (wt) was assessed by DSF in quadruplicate at 100 μM of each compound. Assays were performed in 100 mM HEPES, 150 mM NaCl, pH 7.5, as previously described (https://doi.org/10.1038/nprot.2007.321). T_m (°C) values are presented as mean±SD in the above table. High fluorescence background and weak transitions were observed in the presence of F266A mutant protein. The T_m of the F266A mutant (37.6 ± 0.1 °C) was much lower than the wt-NSD2-PWWP1 (45 ± 0.1 °C).