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. 2000 Feb;66(2):810–815. doi: 10.1128/aem.66.2.810-815.2000

FIG. 1.

FIG. 1

Southern blot analysis of genomic DNAs from T. harzianum wild-type ThzID1 and cotransformant ThzID1-M3. (A) Probed with HindIII-EcoRI fragments from pAN7-2 including the hygromycin B resistance gene. (B) Probed with NcoI-NcoI fragments from pNOM102 including the GUS gene. (C) Probed with HindIII-BamHI fragments from pTEFEGFP including the GFP gene. Lanes: 1, λ-HindIII; 2, 3, and 4: pAN7-2, wild type, and cotransformant, respectively, digested with HindIII-EcoRI; 5, 6, and 7, pNOM102, wild type, and cotransformant, respectively, digested with NcoI-NcoI; 8, 9, and 10, pTEFEGFP, wild type, and cotransformant, respectively, digested with HindIII-BamHI.