Figure 7.

SaTrmK enzymatic activity with RNA^18mer.A, methylation of WT and mutant RNA^18mer by SaTrmK. Each data point of the duplicate measurements is shown (pink and blue). The inset shows the nucleotide sequence and predicted secondary structure of the WT-RNA^18mer. B, methylation of A11-RNA^18mer and 0A-RNA^18mer by SaTrmK. Each data point of the duplicate measurements is shown (pink and blue). The inset shows the nucleotide sequence and predicted secondary structure of the A11-RNA^18mer. C, LC-MS analysis of SaTrmK-catalyzed methylation of A11-RNA^18mer. SaTrmK, S. aureus TrmK; TrmK, m1A22-tRNA methyltransferase.