Figure 3.

Deletion of MHCII in IEC enhances mucosal restitution in DSS colitis. I-Ab^WT and I-Ab^ΔIEC mice were treated with 3% DSS in drinking water for 7 days and allowed to recover for 14 days. Data are representative of 2 independent experiments (n = 6 mice each in DSS group and n = 12–18 mice in H₂O group) (A) Weight loss displayed as percentage relative to the initial weight. (B) Representative hematoxylin-eosin images and violin plots of the colonic inflammation scores (solid lines indicate medians and dashed lines indicate quartiles). Total scores calculated as the sum of proximal and distal colon score (*P < .05, Student t test). (C) qRT-PCR analysis of colonic IL1β, IFNγ, TNFα, and IL22 mRNA in I-Ab^WT and I-Ab^ΔIEC mice. Results normalized to TATA-box binding protein (TBP) mRNA (*P < .05, **P < .01, ****P < .0001; 1-way analysis of variance followed by Bonferroni multiple comparison test). (D) IFNγ, IL1β, and IL22 secretion in colonic explant culture from I-Ab^WT (n = 3) and I-Ab^ΔIEC (n = 6) mice. Values normalized to dry weight of the colonic explants (pg/mL/g). P values from Student t test indicated.