Figure 4.

Constitutive deletion of MHCII expression in IEC is protective in naive T-cell transfer colitis. Colitis was induced in Rag1^−/−I-Ab^WT and Rag1^−/−I-Ab^ΔIEC mice and monitored for 8 weeks. Data are representative of 2 independent experiments (n = 7–11 mice in each group). (A) Weight loss displayed as relative to the initial body weight. *P < .05, **P < .01, Student t test. (B) Violin plots of the colonic inflammation scores (solid lines indicate medians and dashed lines indicate quartiles). (C) qRT-pCr analysis of colonic IL1β, IFNγ, TNFα, and IL12 mRNA in T-cell–transferred Rag1^−/−I-Ab^WT and Rag1^−/−I-Ab^ΔIEC mice. Results normalized to TATA-box binding protein (TBP) mRNA. Statistical significance calculated using 1-way analysis of variance followed by Bonferroni multiple comparison test. *P < .05, **P < .01. (D) Cytokine secretion by colonic explants (Rag1^−/−I-Ab^WT n = 3, Rag1^−/−I-Ab^ΔIEC n = 4). P values of Student t test indicated. (E) Representative flow cytograms of IFNγ-producing CD4⁺ T cells in CD3/CD28-simulated MLN cells. Percentage of CD4⁺IFNγ⁺ cells presented as the summary bar graph. P value of Student t test indicated.